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Genomics and Screening Core Facility

High Throughput Screening and Genomics are some of the most powerful techniques used today to identify new bioactive compounds and determine how cells respond to drug treatment and disease. Researchers tackling cancer, neurodegenerative disorders or infectious diseases and those using regenerative medicine or biomedical engineering to translate research into better patient care, benefit from these established and productive technologies.

For more than 15 years, the University of Galway Genomics and Screening Core Facility has provided researchers with expert training, support and access to high-throughput screening, high-content imaging and qPCR systems, enabling a previously unavailable efficiency, scale and quality of data.

Managed and operated by a dedicated Senior Technical Officer, the Core Facility supports more than 75 researchers annually, across 30 different academic and industry research areas.

Researchers receive specialised training to work autonomously or in collaboration with Core Facility staff, to ensure they are supported in all aspects of data generation and analysis and to maximise the impact of their research.

High-Content Imaging - Operetta (Revvity)

Operetta

Provides automated widefield, brightfield and confocal microscopy for cell-based research
20X, 40X, 60X objectives with full range of excitation and emission filters for unsupervised image acquisition from single slides or multiple plates
Employs Harmony automated image analysis software for phenomic assays, e.g. cell death, cell cycle, cell shape and texture, cell painting, cell migration, 3D analysis, protein quantification and localisation.

High-Throughput Screening - Janus Automated Workstation (Revvity)

Janus

Enables automated, flexible and high-throughput liquid handling to 96 and 384 well plates.
Operations include sample preparation, mixing, dilution, cell seeding, treatment, fixation and staining.
Class II biosafety cabinet and integrated gripper arm ensure sterile plate handling for cell-based assays.

High-Throughput Screening – Victor X5 Multilabel Plate Reader (Revvity)

Enables automated and high-throughput absorbance, fluorescence and luminescence assay detection in 96 and 384 well plates.
Stacker module enables unsupervised processing of up 20 plates.

High-Throughput Screening – Multiflo FX (Biotek)

Multiflo

Enables low-volume, high-speed dispensing to 96 and 384 well plates and deep well plates.
No tips required, reducing cost and lab plastic consumption.

qPCR – QuantStudio 5 (Applied Biosystems)

Enables low volume gene expression, SNP and melt curve analysis in 96 well plates.
Provides 10 logs linear dynamic range for increased assay sensitivity.