Home ▻ Colleges & Schools▻College of Science and Engineering▻Technology Services Directorate Galway▻Genomics and Screening

Genomics and Screening

Overview

High Throughput Screening and Genomics are some of the most cutting-edge techniques used today to identify new bioactive compounds and determine how cells respond to drug treatment and disease. Researchers tackling cancer, neurodegenerative disorders or infectious diseases and those using regenerative medicine or biomedical engineering to translate research into better patient care, benefit from these established and productive technologies.

For more than 15 years, University of Galway Genomics and Screening Core Facility has provided researchers with expert training, support and access to high-throughput screening, high-content imaging and qPCR systems, enabling a previously unavailable efficiency, scale and quality of data.

Genomics and Screen Core Facility Equipment

Revvity Operetta

Type: High Content Imaging

  • Automated imaging of multiwell plates, slides, etc.
  • Widefield, brightfield and confocal microscopy
  • Automated image analysis, e.g: cell painting, cell death, cell cycle, migration, 3D analysis, protein quantification and localisation

Revvity Janus

Type: High Throughput Screening Workstation

  • Automated high-throughput liquid handling to multiwell plates for cell-based assays
  • Operations include: sample preparation, dilution, cell seeding, treatment, fixation and staining

Biotek Multiflo FX

Type: Automated Dispenser

  • Rapid automated reagent dispensing to multiwell plates for cell-based assays, ELISAs, etc.

ABI QuantStudio 5 & QuantStudio 3

Type: Quantitative polymerase chain reaction

  • Gene and miRNA expression, genotyping, melt curve analysis
  • 4-6 colour multiplexing
  • VeriFlex temp. optimisation

ABI Veriti

Type: Polymerase chain reaction

  • Endpoint PCR
  • cDNA synthesis

Agilent TapeStation 4150

Type: Nucleic Acid QC

  • DNA and RNA analysis of size, concentration and integrity as part of NGS workflow

BMG Labtech VANTAStar

Type: MultimodePlate Reader

  • Fluorescence, absorbance, luminescence and fluorescent polarisation assays possible
  • Monochromator for 220-1000 nm
  • ELISAs, protein conc., cell viability, cytotoxicity, enzyme activity, reporter assays, binding assay

10x Chromium

Type: Single cell sequencer

Single cell sequencing allows scientists to see the unique gene expression patterns of each cell. This view more fully characterizes tissue heterogeneity, revealing the rare cell types that have big consequences in health and disease.

Key Publications from this Core Facility

Lambert, R. et al. (2025) ‘Drug-induced cytotoxicity prediction in muscle cells, an application of the cell painting assay’, PLOS ONE, 20(3). doi:10.1371/journal.pone.0320040.

Alsharabasy, A.M. et al. (2023) ‘Nitric oxide-scavenging, anti-migration effects, and glycosylation changes after hemin treatment of human triple-negative breast cancer cells: A mechanistic study’, ACS Pharmacology & Translational Science, 6(10), pp. 1416–1432. doi:10.1021/acsptsci.3c00115.

Marchese, P. et al. (2021) ‘Deep-sea coral garden invertebrates and their associated fungi are genetic resources for Chronic Disease Drug Discovery’, Marine Drugs, 19(7), p. 390. doi:10.3390/md19070390.

Patil, V. et al. (2021) ‘A robust platform for high-throughput screening of therapeutic strategies for acute and Chronic Spinal Cord Injury’, iScience, 24(3), p. 102182. doi:10.1016/j.isci.2021.102182.

Tashakor, A. et al. (2019) ‘A new split‐luciferase complementation assay identifies pentachlorophenol as an inhibitor of Apoptosome Formation’, FEBS Open Bio, 9(7), pp. 1194–1203. doi:10.1002/2211-5463.12646.

For more information, please contact genomics@universityofgalway.ie.