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Dr Krystyna Cwiklinski

B.Sc., M.Sc., PhD

 
researcher
 

Biography

Dr Krystyna Cwiklinski received her PhD in Molecular Biology studying Trichinella serine proteases from the University of Aberdeen, Scotland. Following her PhD, Krystyna carried out post-doctoral work at the University of Liverpool, focussed on cyathostomin horse nematodes and the economically important pathogen of livestock (sheep and cattle) and people, the liver fluke, Fasciola hepatica. Using a combination of classical gene-targeted approaches and ‘omics’ technologies Krystyna has created many -omics datasets which she interrogates to investigate drug resistance, parasite development and host-parasite interaction to discover novel targets for vaccines and diagnostics. Krystyna joined NUI Galway in April 2019 as a Research Fellow continuing work started at Queen’s University, Belfast in the Molecular Parasitology Laboratory, focussed on developing ‘omics’-inspired vaccines for F. hepatica. She works closely with Teagasc, Athenry, Galway, in the evaluation of new vaccines in large animal trials (sheep and cattle).

http://www.nuigalway.ie/science-engineering/school-of-natural-sciences/disciplines/zoology/research/molecularparasitology/

Peer Reviewed Journals

  Year Publication
(2021) 'Biochemical and cellular characterisation of the Plasmodium falciparum M1 alanyl aminopeptidase (Pf M1AAP) and M17 leucyl aminopeptidase (Pf M17LAP)'
Rency Mathew, Juliane Wunderlich, Karine Thivierge, Krystyna Cwiklinski, Claire Dumont, Leann Tilley, Petra Rohrbach, John P Dalton (2021) 'Biochemical and cellular characterisation of the Plasmodium falciparum M1 alanyl aminopeptidase (Pf M1AAP) and M17 leucyl aminopeptidase (Pf M17LAP)'. Scientific Reports, [ARAN Link] [Details]
(2021) 'Complementary transcriptomic and proteomic analyses reveal the cellular and molecular processes that drive growth and development of Fasciola hepatica in the host liver'
Krystyna Cwiklinski , Mark W Robinson, Sheila Donnelly, John P Dalton (2021) 'Complementary transcriptomic and proteomic analyses reveal the cellular and molecular processes that drive growth and development of Fasciola hepatica in the host liver'. BMC Genomics, [DOI] [ARAN Link] [Details]
(2021) 'Eudiplozoon nipponicum (Monogenea, Diplozoidae) and its adaptation to haematophagy as revealed by transcriptome and secretome profiling'
Vorel J;Cwiklinski K;Roudnický P;Ilgová J;Jedli¿ková L;Dalton JP;Mike¿ L;Gelnar M;Ka¿ný M; (2021) 'Eudiplozoon nipponicum (Monogenea, Diplozoidae) and its adaptation to haematophagy as revealed by transcriptome and secretome profiling'. BMC Genomics, 22 (1) [DOI] [Details]
(2021) 'Fasciola hepatica hijacks host macrophage miRNA machinery to modulate early innate immune responses'
Tran N;Ricafrente A;To J;Lund M;Marques TM;Gama-Carvalho M;Cwiklinski K;Dalton JP;Donnelly S; (2021) 'Fasciola hepatica hijacks host macrophage miRNA machinery to modulate early innate immune responses'. Scientific Reports, 11 (1) [DOI] [Details]
(2020) 'Fasciola hepaticaSerine Protease Inhibitor Family (Serpins): Purposely crafted for regulating host proteases'
Verissimo, CD;Jewhurst, HL;Tikhonova, IG;Urbanus, RT;Maule, AG;Dalton, JP;Cwiklinski, K (2020) 'Fasciola hepaticaSerine Protease Inhibitor Family (Serpins): Purposely crafted for regulating host proteases'. Plos Neglected Tropical Diseases, 14 [DOI] [Details]
(2020) 'Regulation of the Fasciola hepatica newly excysted juvenile cathepsin L3 (FhCL3) by its propeptide: a proposed 'clamp-like' mechanism of binding and inhibition'
Pritsch IC;Tikhonova IG;Jewhurst HL;Drysdale O;Cwiklinski K;Molento MB;Dalton JP;Verissimo CM; (2020) 'Regulation of the Fasciola hepatica newly excysted juvenile cathepsin L3 (FhCL3) by its propeptide: a proposed 'clamp-like' mechanism of binding and inhibition'. Bmc Molecular And Cell Biology, 21 (1) [DOI] [Details]
(2020) 'An atypical and functionally diverse family of Kunitz-type cysteine/serine proteinase inhibitors secreted by the helminth parasite Fasciola hepatica'
Smith D;Cwiklinski K;Jewhurst H;Tikhonova IG;Dalton JP; (2020) 'An atypical and functionally diverse family of Kunitz-type cysteine/serine proteinase inhibitors secreted by the helminth parasite Fasciola hepatica'. Scientific Reports, 10 (1) [DOI] [ARAN Link] [Details]
(2020) 'Fasciola hepatica Extracellular Vesicles isolated from excretory-secretory products using a gravity flow method modulate dendritic cell phenotype and activity'
Murphy A;Cwiklinski K;Lalor R;O'Connell B;Robinson MW;Gerlach J;Joshi L;Kilcoyne M;Dalton JP;O'Neill SM; (2020) 'Fasciola hepatica Extracellular Vesicles isolated from excretory-secretory products using a gravity flow method modulate dendritic cell phenotype and activity'. Plos Neglected Tropical Diseases, 14 (9) [DOI] [Details]
(2020) 'Schistosoma mansoni immunomodulatory molecule Sm16/SPO-1/SmSLP is a member of the trematode-specific helminth defence molecules (HDMs)'
Shiels J;Cwiklinski K;Alvarado R;Thivierge K;Cotton S;Gonzales Santana B;To J;Donnelly S;Taggart CC;Weldon S;Dalton JP; (2020) 'Schistosoma mansoni immunomodulatory molecule Sm16/SPO-1/SmSLP is a member of the trematode-specific helminth defence molecules (HDMs)'. Plos Neglected Tropical Diseases, 14 (7) [DOI] [ARAN Link] [Details]
(2019) 'The cathepsin-like cysteine peptidases of trematodes of the genus Fasciola'
Cwiklinski K;Donnelly S;Drysdale O;Jewhurst H;Smith D;De Marco Verissimo C;Pritsch IC;O'Neill S;Dalton JP;Robinson MW; (2019) 'The cathepsin-like cysteine peptidases of trematodes of the genus Fasciola'. Advances In Parasitology, 104 [DOI] [Details]
(2019) 'Surface molecules of extracellular vesicles secreted by the helminth pathogen Fasciola hepatica direct their internalisation by host cells'
de la Torre-Escudero, E,Gerlach, JQ,Bennett, APS,Cwiklinski, K,Jewhurst, HL,Huson, KM,Joshi, L,Kilcoyne, M,O'Neill, S,Dalton, JP,Robinson, MW (2019) 'Surface molecules of extracellular vesicles secreted by the helminth pathogen Fasciola hepatica direct their internalisation by host cells'. Plos Neglected Tropical Diseases, 13 [DOI] [Details]
(2019) 'Complex and dynamic transcriptional changes allow the helminth Fasciola gigantica to adjust to its intermediate snail and definitive mammalian hosts'
Zhang XX;Cwiklinski K;Hu RS;Zheng WB;Sheng ZA;Zhang FK;Elsheikha HM;Dalton JP;Zhu XQ; (2019) 'Complex and dynamic transcriptional changes allow the helminth Fasciola gigantica to adjust to its intermediate snail and definitive mammalian hosts'. BMC Genomics, 20 (1) [DOI] [ARAN Link] [Details]
(2018) 'Advances in Fasciola hepatica research using 'omics' technologies'
Cwiklinski K;Dalton JP; (2018) 'Advances in Fasciola hepatica research using 'omics' technologies'. International Journal For Parasitology, 48 (5) [DOI] [Details]
(2018) 'Low allelic diversity in vaccine candidates genes from different locations sustain hope for Fasciola hepatica immunization'
Dominguez MF;González-Miguel J;Carmona C;Dalton JP;Cwiklinski K;Tort J;Siles-Lucas M; (2018) 'Low allelic diversity in vaccine candidates genes from different locations sustain hope for Fasciola hepatica immunization'. Veterinary Parasitology, 258 [DOI] [Details]
(2018) 'Clonal amplification of Fasciola hepatica in Galba truncatula: within and between isolate variation of triclabendazole-susceptible and -resistant clones'
Hodgkinson JE;Cwiklinski K;Beesley N;Hartley C;Allen K;Williams DJL; (2018) 'Clonal amplification of Fasciola hepatica in Galba truncatula: within and between isolate variation of triclabendazole-susceptible and -resistant clones'. Parasites & vectors, 11 (1) [DOI] [Details]
(2018) 'Infection by the Helminth Parasite Fasciola hepatica Requires Rapid Regulation of Metabolic, Virulence, and Invasive Factors to Adjust to Its Mammalian Host'
Cwiklinski K;Jewhurst H;McVeigh P;Barbour T;Maule AG;Tort J;O'Neill SM;Robinson MW;Donnelly S;Dalton JP; (2018) 'Infection by the Helminth Parasite Fasciola hepatica Requires Rapid Regulation of Metabolic, Virulence, and Invasive Factors to Adjust to Its Mammalian Host'. Molecular & Cellular Proteomics, 17 (4) [DOI] [Details]
(2018) 'In silico analyses of protein glycosylating genes in the helminth Fasciola hepatica (liver fluke) predict protein-linked glycan simplicity and reveal temporally-dynamic expression profiles'
McVeigh P;Cwiklinski K;Garcia-Campos A;Mulcahy G;O'Neill SM;Maule AG;Dalton JP; (2018) 'In silico analyses of protein glycosylating genes in the helminth Fasciola hepatica (liver fluke) predict protein-linked glycan simplicity and reveal temporally-dynamic expression profiles'. Scientific Reports, 8 (1) [DOI] [Details]
(2017) 'Immune signatures of pathogenesis in the peritoneal compartment during early infection of sheep with Fasciola hepatica'
Ruiz-Campillo MT;Molina Hernandez V;Escamilla A;Stevenson M;Perez J;Martinez-Moreno A;Donnelly S;Dalton JP;Cwiklinski K; (2017) 'Immune signatures of pathogenesis in the peritoneal compartment during early infection of sheep with Fasciola hepatica'. Scientific Reports, 7 (1) [DOI] [Details]
(2016) 'Tegument Glycoproteins and Cathepsins of Newly Excysted Juvenile Fasciola hepatica Carry Mannosidic and Paucimannosidic N-glycans'
Garcia-Campos A;Ravidà A;Nguyen DL;Cwiklinski K;Dalton JP;Hokke CH;O'Neill S;Mulcahy G; (2016) 'Tegument Glycoproteins and Cathepsins of Newly Excysted Juvenile Fasciola hepatica Carry Mannosidic and Paucimannosidic N-glycans'. Plos Neglected Tropical Diseases, 10 (5) [DOI] [Details]
(2016) 'A prospective view of animal and human Fasciolosis'
Cwiklinski K;O'Neill SM;Donnelly S;Dalton JP; (2016) 'A prospective view of animal and human Fasciolosis'. Parasite Immunology, 38 (9) [DOI] [Details]
(2016) 'Unexpected Activity of a Novel Kunitz-type Inhibitor: INHIBITION OF CYSTEINE PROTEASES BUT NOT SERINE PROTEASES'
Smith D;Tikhonova IG;Jewhurst HL;Drysdale OC;Dvo¿ák J;Robinson MW;Cwiklinski K;Dalton JP; (2016) 'Unexpected Activity of a Novel Kunitz-type Inhibitor: INHIBITION OF CYSTEINE PROTEASES BUT NOT SERINE PROTEASES'. Journal Of Biological Chemistry, 291 (37) [DOI] [Details]
(2016) 'Fasciola hepatica surface tegument: glycoproteins at the interface of parasite and host'
Ravida A;Cwiklinski K;Aldridge AM;Clarke P;Thompson R;Gerlach JQ;Kilcoyne M;Hokke CH;Dalton JP;O'Neill SM; (2016) 'Fasciola hepatica surface tegument: glycoproteins at the interface of parasite and host'. Molecular & Cellular Proteomics : Mcp, [DOI] [Details]
(2015) 'Characterisation of a novel panel of polymorphic microsatellite loci for the liver fluke, Fasciola hepatica, using a next generation sequencing approach'
Cwiklinski K;Allen K;LaCourse J;Williams DJ;Paterson S;Hodgkinson JE; (2015) 'Characterisation of a novel panel of polymorphic microsatellite loci for the liver fluke, Fasciola hepatica, using a next generation sequencing approach'. Infection Genetics And Evolution, 32 [DOI] [Details]
(2015) 'The Fasciola hepatica genome: gene duplication and polymorphism reveals adaptation to the host environment and the capacity for rapid evolution'
Cwiklinski K;Dalton JP;Dufresne PJ;La Course J;Williams DJ;Hodgkinson J;Paterson S; (2015) 'The Fasciola hepatica genome: gene duplication and polymorphism reveals adaptation to the host environment and the capacity for rapid evolution'. Genome Biology, 16 [DOI] [Details]
(2015) 'Fasciola hepatica vaccine: we may not be there yet but we're on the right road'
Molina-Hernández V;Mulcahy G;Pérez J;Martínez-Moreno Á;Donnelly S;O'Neill SM;Dalton JP;Cwiklinski K; (2015) 'Fasciola hepatica vaccine: we may not be there yet but we're on the right road'. Veterinary Parasitology, 208 (1-2) [DOI] [Details]
(2015) 'The Extracellular Vesicles of the Helminth Pathogen, Fasciola hepatica: Biogenesis Pathways and Cargo Molecules Involved in Parasite Pathogenesis'
Cwiklinski K;de la Torre-Escudero E;Trelis M;Bernal D;Dufresne PJ;Brennan GP;O'Neill S;Tort J;Paterson S;Marcilla A;Dalton JP;Robinson MW; (2015) 'The Extracellular Vesicles of the Helminth Pathogen, Fasciola hepatica: Biogenesis Pathways and Cargo Molecules Involved in Parasite Pathogenesis'. Molecular & Cellular Proteomics, 14 (12) [DOI] [Details]
(2015) 'Fasciola hepatica from naturally infected sheep and cattle in Great Britain are diploid'
Beesley NJ;Cwiklinski K;Williams DJ;Hodgkinson J; (2015) 'Fasciola hepatica from naturally infected sheep and cattle in Great Britain are diploid'. Journal Of Parasitology, 142 (9) [DOI] [Details]
(2013) 'Identification of putative markers of triclabendazole resistance by a genome-wide analysis of genetically recombinant Fasciola hepatica'
Hodgkinson J;Cwiklinski K;Beesley NJ;Paterson S;Williams DJ; (2013) 'Identification of putative markers of triclabendazole resistance by a genome-wide analysis of genetically recombinant Fasciola hepatica'. Journal Of Parasitology, 140 (12) [DOI] [Details]
(2013) 'Transcriptome analysis of a parasitic clade V nematode: comparative analysis of potential molecular anthelmintic targets in Cylicostephanus goldi'
Cwiklinski K;Merga JY;Lake SL;Hartley C;Matthews JB;Paterson S;Hodgkinson JE; (2013) 'Transcriptome analysis of a parasitic clade V nematode: comparative analysis of potential molecular anthelmintic targets in Cylicostephanus goldi'. International Journal For Parasitology, 43 (11) [DOI] [Details]
(2012) 'New insights into sequence variation in the IGS region of 21 cyathostomin species and the implication for molecular identification'
Cwiklinski K;Kooyman FN;Van Doorn DC;Matthews JB;Hodgkinson JE; (2012) 'New insights into sequence variation in the IGS region of 21 cyathostomin species and the implication for molecular identification'. Journal Of Parasitology, 139 (8) [DOI] [Details]
(2009) 'Cloning and analysis of a Trichinella pseudospiralis muscle larva secreted serine protease gene'
Cwiklinski K;Meskill D;Robinson MW;Pozio E;Appleton JA;Connolly B; (2009) 'Cloning and analysis of a Trichinella pseudospiralis muscle larva secreted serine protease gene'. Veterinary Parasitology, 159 (3-4) [DOI] [Details]