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Biological and Chemical Sciences

Prof Corrado Santocanale

Ph.D

Contact Details

Professor
SCHOOL OF NATURAL SCIENCES
Room BMS-G025 ground floor
Biomedical Sciences Building
North Campus
T: Ext. 5174
E: corrado.santocanale@universityofgalway.ie
 
researcher
 

Biography

Professor Corrado Santocanale received his Ph.D. in Cellular and Molecular Biology at the University of Milan, Italy, in 1993. His early work focused on the characterization of enzymes required for the duplication of the DNA in the yeast S. cerevisiae. He was then awarded an EU Marie Curie postdoctoral fellowship to work on the molecular mechanisms that control initiation of DNA replication at the Clare Hall Laboratories, Cancer Research UK, London. He spent eight years in the pharmaceutical industry at the Oncology R&D site in Nerviano, Italy (former Pharmacia/Pfizer and now Nerviano Medical Sciences) both as a group and as a project leader developing protein kinase inhibitors for the treatment of human cancers. He returned to academia  in 2007. The major achievement in Cancer Drug Discovery was to propose and lead a program through all the stages of the pre-clinical drug discovery process delivering a compound with a novel mechanism of action that entered clinical trials. The major achievements in basic cancer research include: - Identification and characterization of the "origin firing checkpoint", a biochemical pathway that is activated in response to the inhibition of DNA replication elongation thus preventing the activation of new replication origins. - Identification of the second "cyclin like" regulatory subunit of the human Cdc7 kinase. - The identification of a biochemical pathway, altered in cancer cells, responding to the inhibition of the Cdc7 kinase. Identification of the gene encoding the first eukaryotic DNA primase large subunit. 

Research Interests

The process of DNA replication is the biggest threat for genome stability in all proliferating cells. Cancer cells in particular are subjected to replication stress after activation of proto-oncogenes into their oncogenic forms or due to deficiency in specific factors required for genome duplication. Thus aberrant DNA replication contributes to initiating and maintaining the cancerous state, while drugs targeting DNA synthesis have potent antitumor activity and are key component of current and novel chemotherapeutic regimens.

Our laboratory is studying the mechanisms that regulate genome replication in human cells with particular emphasis on the Cell Division Cycle 7 kinase (CDC7). CDC7 acts as a molecular switch for DNA synthesis and is also thought to participate in several other processes that regulate normal cell cycle progression and chromosome dynamics.We have adopted chemical biology and gene editing approaches to characterize the molecular processes in which CDC7 is involved and to detect new substrates of the kinase. We are performing genome-wide CRISPR/Cas9 screens to identify genes that alter the cellular responses and make cells more sensitive or resistant to CDC7 inhibition.This information will be pivotal in understanding in what disease context emerging CDC7 inhibitors may be used for the cancer treatment, and in developing novel biomarkers, thus providing valuable information and tools for rationally driving patient selection and devising combination therapies in preclinical and clinical settings.

We are also interested in understanding the roles of the Ubiquitin Specific Protease 9X (USP9X) which in context dependent manner can act as either an oncogene or a tumour suppressor. We have recently discovered that USP9X contributes to genome stability by promoting forks stability and efficient DNA repair, but by different mechanisms. 

In the past we have established a technique for the purification and characterization of proteins associated to newly replicated DNA that we called DNA mediated chromatin pull down (Dm-ChP).  This technique has been particularly useful in understanding which proteins acts at forks during unperturbed replication, upon replication stress and in different genetic backgrounds.

Cell Cycle, DNA replication, Cancer therapeutics
 

Research Projects

  Project Start Date End Date
IRC POSTGRADUATE SCHOLARSHIP MARK McDERMOTT _ ROLE OF CDC7 AND ITS REGULATORY SUBUNITS AT STALLED REPLICATION FORKS 01-SEP-19 31-AUG-22
US-Ireland R&D Partnership Planning Grant - Cancer Research 15-MAR-18 15-SEP-19
UNCOVERING THE FUNDAMENTAL ROLES OF THE CDC7 KINASE AND OF ITS REGULATORY SUBUNITS THROUGH GENOME EDITING TECHNOLOGY 01-NOV-17 31-OCT-21
SANTOCANALE MOLECULAR MEDICINE 20-MAY-09 31-DEC-20
Cellular responses to ATP competitive CDC7 kinase inhibitors 01-FEB-16 31-JAN-23
THERAPEUTIC MICRO RNA CONTROL OF PROSTATE CANCER GROWTH IRISH CANCER SOCIETY PCI11BAR CORRADO SANTOCANALE 01-JUL-12 31-DEC-13
CDC7 REGULATION OF CLASPIN STABILITY AND ITS EFFECT ON CELL CYCLE CHECKPOINT FUNCTION C SANTOCANALE 01-APR-13 31-DEC-19
CDC7 AND CLASPIN IN THE REGULATION OF DNA REPLICATION IN BREAST CANCER CELLS BREAST CANCER CAMPAIGN CORRADO SANTOCANALE 01-NOV-12 31-JAN-16
GUAN NAN WANG IRCSET EMPOWER POSTDOC CORRADO SANTOCANALE 01-JUL-12 19-OCT-15
ESI HEA PRTLI5 MMI CTRSP EDEL MCGARRY 01-MAR-11 25-OCT-18
EHA ASH RESEARCH EXCH AWRD MARK COYNE 01-JUL-10 30-NOV-12
UNDERSTAND EXPLOIT MOLECULAR DNA 08/IN.1/B2064 - C SANTOCANALE 01-SEP-09 05-JAN-17
REGULATION BY MICRORNAS AND ITS IMPACT IN CANCER 01-OCT-08 30-SEP-12
SFI CDC7 KINASE IN CANCER CELLS EXPOSED TO S-PHASE 01-SEP-08 31-AUG-11

Contract Researchers

  Researcher Name Project Role Funding Body
Michael Rainey Roles of CDC7 kinase Post-doctoral researcher Science Foundation Ireland (SFI)

Peer Reviewed Journals

  Year Publication
(2024) 'DBF4, not DRF1, is the crucial regulator of CDC7 kinase at replication forks'
Anja Göder, Chrystelle Antoinat Maric, Michael D Rainey, Aisling O'Connor, Chiara Cazzaniga, Daniel Shamavu, Jean-Charles Cadoret, Corrado Santocanale (2024) 'DBF4, not DRF1, is the crucial regulator of CDC7 kinase at replication forks'. J Cell Biol, 223 (8) [DOI] [Details]
(2024) 'CDC7 inhibition drives an inflammatory response and a p53-dependent senescent-like state in breast epithelial cells'
Chiara Cazzaniga; Anja Göder; Michael David Rainey; Aisling Quinlan; Simone Coughlan; Stefanus Bernard; Corrado Santocanale (2024) 'CDC7 inhibition drives an inflammatory response and a p53-dependent senescent-like state in breast epithelial cells'. Febs Journal, 291 (14):3147-3168 [DOI] [Details]
(2023) 'PTBP1 Enforces ATR-CHK1 Signalling Determining the Potency of CDC7 Inhibitors'
Göder, A., Quinlan, A., Rainey, M.D., Bennett, D., Shamavu, D., Corso, J., and Santocanale, C. (2023) 'PTBP1 Enforces ATR-CHK1 Signalling Determining the Potency of CDC7 Inhibitors'. Iscience, [Details]
(2020) 'CDC7 kinase promotes MRE11 fork processing, modulating fork speed and chromosomal breakage'
Rainey MD, Quinlan A, Cazzaniga C, Mijic S, Martella O, Krietsch J, Göder A, Lopes M, Santocanale C (2020) 'CDC7 kinase promotes MRE11 fork processing, modulating fork speed and chromosomal breakage'. Embo Rep, [DOI] [Details]
(2020) 'ATR Restrains DNA Synthesis and Mitotic Catastrophe in Response to CDC7 Inhibition'
Rainey, M. D. and Bennett, D. and O'Dea, R. and Zanchetta, M. E. and Voisin, M. and Seoighe, C. and Santocanale, C. (2020) 'ATR Restrains DNA Synthesis and Mitotic Catastrophe in Response to CDC7 Inhibition'. Cell Rep, 32 (9) [DOI] [Details]
(2018) 'Repression of Mcl-1 expression by the CDC7/CDK9 inhibitor PHA-767491 overcomes bone marrow stroma-mediated drug resistance in AML'
E, O' Reilly and Dhami, S. P. S. and Baev, D. V. and Ortutay, C. and Halpin-McCormick, A. and Morrell, R. and Santocanale, C. and Samali, A. and Quinn, J. and O'Dwyer, M. E. and Szegezdi, E. (2018) 'Repression of Mcl-1 expression by the CDC7/CDK9 inhibitor PHA-767491 overcomes bone marrow stroma-mediated drug resistance in AML'. Sci Rep, 8 (1) [DOI] [Details]
(2017) 'DNA Replication Dynamics and Cellular Responses to ATP Competitive CDC7 Kinase Inhibitors'
Rainey, M. D. and Quachthithu, H. and Gaboriau, D. and Santocanale, C. (2017) 'DNA Replication Dynamics and Cellular Responses to ATP Competitive CDC7 Kinase Inhibitors'. ACS Chem Biol, 12 (7):1893-1902 [DOI] [Details]
(2015) 'Synthesis of Migrastatin Analogues as Inhibitors of Tumour Cell Migration: Exploring Structural Change in and on the Macrocyclic Ring'
Lo Re, D. and Zhou, Y. and Mucha, J. and Jones, L. F. and Leahy, L. and Santocanale, C. and Krol, M. and Murphy, P. V. (2015) 'Synthesis of Migrastatin Analogues as Inhibitors of Tumour Cell Migration: Exploring Structural Change in and on the Macrocyclic Ring'. Chemistry, 21 (50):18109-21 [DOI] [Details]
(2015) 'Synthesis of Migrastatin Analogues as Inhibitors of Tumour Cell Migration: Exploring Structural Change in and on the Macrocyclic Ring'
Lo Re, D. and Zhou, Y. and Mucha, J. and Jones, L. F. and Leahy, L. and Santocanale, C. and Krol, M. and Murphy, P. V. (2015) 'Synthesis of Migrastatin Analogues as Inhibitors of Tumour Cell Migration: Exploring Structural Change in and on the Macrocyclic Ring'. Chemistry, 21 (50) [DOI] [Details]
(2014) 'A high through-put screen for small molecules modulating MCM2 phosphorylation identifies Ryuvidine as an inducer of the DNA damage response'
FitzGerald, J. and Murillo, L. S. and O'Brien, G. and O'Connell, E. and O'Connor, A. and Wu, K. and Wang, G. N. and Rainey, M. D. and Natoni, A. and Healy, S. and O'Dwyer, M. and Santocanale, C. (2014) 'A high through-put screen for small molecules modulating MCM2 phosphorylation identifies Ryuvidine as an inducer of the DNA damage response'. PLoS One, 9 (6) [DOI] [Details]
(2013) 'Cell cycle-dependent formation of Cdc45-Claspin complexes in human cells is compromized by UV-mediated DNA damage'
Broderick, R. and Rainey, M. D. and Santocanale, C. and Nasheuer, H. P. (2013) 'Cell cycle-dependent formation of Cdc45-Claspin complexes in human cells is compromized by UV-mediated DNA damage'. FEBS J, 280 (19):4888-902 [DOI] [Details]
(2013) 'Characterization of a Dual CDC7/CDK9 Inhibitor in Multiple Myeloma Cellular Models'
Natoni, A. and Coyne, M. R. and Jacobsen, A. and Rainey, M. D. and O'Brien, G. and Healy, S. and Montagnoli, A. and Moll, J. and O'Dwyer, M. and Santocanale, C. (2013) 'Characterization of a Dual CDC7/CDK9 Inhibitor in Multiple Myeloma Cellular Models'. Cancers (Basel), 5 (3):901-18 [DOI] [Details]
(2013) 'Cdc7-dependent and -independent phosphorylation of Claspin in the induction of the DNA replication checkpoint'
Rainey, M. D. and Harhen, B. and Wang, G. N. and Murphy, P. V. and Santocanale, C. (2013) 'Cdc7-dependent and -independent phosphorylation of Claspin in the induction of the DNA replication checkpoint'. Cell Cycle, 12 (10):1560-8 [DOI] [Details]
(2013) 'MicroRNA-29a regulates the benzo[a]pyrene dihydrodiol epoxide-induced DNA damage response through Cdc7 kinase in lung cancer cells'
Barkley, L. R. and Santocanale, C. (2013) 'MicroRNA-29a regulates the benzo[a]pyrene dihydrodiol epoxide-induced DNA damage response through Cdc7 kinase in lung cancer cells'. Oncogenesis, 2 (7) [DOI] [Details]
(2013) 'A cell culture system that mimics chronic lymphocytic leukemia cells microenvironment for drug screening and characterization'
Natoni, A. and O'Dwyer, M. and Santocanale, C. (2013) 'A cell culture system that mimics chronic lymphocytic leukemia cells microenvironment for drug screening and characterization'. Methods Mol Biol, 986 :217-26 [DOI] [Details]
(2012) 'Hypoxic activation of ATR and the suppression of the initiation of DNA replication through cdc6 degradation'
Martin, L. and Rainey, M. and Santocanale, C. and Gardner, L. B. (2012) 'Hypoxic activation of ATR and the suppression of the initiation of DNA replication through cdc6 degradation'. Oncogene, 31 (36):4076-84 [DOI] [Details]
(2011) 'DNA mediated chromatin pull-down for the study of chromatin replication'
Kliszczak, A. E. and Rainey, M. D. and Harhen, B. and Boisvert, F. M. and Santocanale, C. (2011) 'DNA mediated chromatin pull-down for the study of chromatin replication'. Sci Rep, 1 [DOI] [Details]
(2011) 'Isosteric replacement of the Z-enone with haloethyl ketone and E-enone in a resorcylic acid lactone series and biological evaluation'
Napolitano, C. and Natoni, A. and Santocanale, C. and Evensen, L. and Lorens, J. B. and Murphy, P. V. (2011) 'Isosteric replacement of the Z-enone with haloethyl ketone and E-enone in a resorcylic acid lactone series and biological evaluation'. Bioorg Med Chem Lett, 21 (4):1167-70 [DOI] [Details]
(2010) 'Cdc7 kinase inhibitors: 5-heteroaryl-3-carboxamido-2-aryl pyrroles as potential antitumor agents. 1. Lead finding'
Menichincheri, M. and Albanese, C. and Alli, C. and Ballinari, D. and Bargiotti, A. and Caldarelli, M. and Ciavolella, A. and Cirla, A. and Colombo, M. and Colotta, F. and Croci, V. and D'Alessio, R. and D'Anello, M. and Ermoli, A. and Fiorentini, F. and Forte, B. and Galvani, A. and Giordano, P. and Isacchi, A. and Martina, K. and Molinari, A. and Moll, J. K. and Montagnoli, A. and Orsini, P. and Orzi, F. and Pesenti, E. and Pillan, A. and Roletto, F. and Scolaro, A. and Tato, M. and Tibolla, M. and Valsasina, B. and Varasi, M. and Vianello, P. and Volpi, D. and Santocanale, C. and Vanotti, E. (2010) 'Cdc7 kinase inhibitors: 5-heteroaryl-3-carboxamido-2-aryl pyrroles as potential antitumor agents. 1. Lead finding'. J Med Chem, 53 (20):7296-315 [DOI] [Details]
(2010) 'Cdc7 kinase - a new target for drug development'
Swords, R. and Mahalingam, D. and O'Dwyer, M. and Santocanale, C. and Kelly, K. and Carew, J. and Giles, F. (2010) 'Cdc7 kinase - a new target for drug development'. Eur J Cancer, 46 (1):33-40 [DOI] [Details]
(2009) 'Cell division cycle 7 kinase inhibitors: 1H-pyrrolo[2,3-b]pyridines, synthesis and structure-activity relationships'
Ermoli, A. and Bargiotti, A. and Brasca, M. G. and Ciavolella, A. and Colombo, N. and Fachin, G. and Isacchi, A. and Menichincheri, M. and Molinari, A. and Montagnoli, A. and Pillan, A. and Rainoldi, S. and Sirtori, F. R. and Sola, F. and Thieffine, S. and Tibolla, M. and Valsasina, B. and Volpi, D. and Santocanale, C. and Vanotti, E. (2009) 'Cell division cycle 7 kinase inhibitors: 1H-pyrrolo[2,3-b]pyridines, synthesis and structure-activity relationships'. J Med Chem, 52 (14):4380-90 [DOI] [Details]
(2009) 'Evidence for the progression through S-phase in the ectopic cell cycle re-entry of neurons in Alzheimer disease'
Bonda, D. J. and Evans, T. A. and Santocanale, C. and Llosa, J. C. and Vina, J. and Bajic, V. and Castellani, R. J. and Siedlak, S. L. and Perry, G. and Smith, M. A. and Lee, H. G. (2009) 'Evidence for the progression through S-phase in the ectopic cell cycle re-entry of neurons in Alzheimer disease'. Aging (Albany NY), 1 (4):382-8 [DOI] [Details]
(2009) 'First Cdc7 kinase inhibitors: pyrrolopyridinones as potent and orally active antitumor agents. 2. Lead discovery'
Menichincheri, M. and Bargiotti, A. and Berthelsen, J. and Bertrand, J. A. and Bossi, R. and Ciavolella, A. and Cirla, A. and Cristiani, C. and Croci, V. and D'Alessio, R. and Fasolini, M. and Fiorentini, F. and Forte, B. and Isacchi, A. and Martina, K. and Molinari, A. and Montagnoli, A. and Orsini, P. and Orzi, F. and Pesenti, E. and Pezzetta, D. and Pillan, A. and Poggesi, I. and Roletto, F. and Scolaro, A. and Tato, M. and Tibolla, M. and Valsasina, B. and Varasi, M. and Volpi, D. and Santocanale, C. and Vanotti, E. (2009) 'First Cdc7 kinase inhibitors: pyrrolopyridinones as potent and orally active antitumor agents. 2. Lead discovery'. J Med Chem, 52 (2):293-307 [DOI] [Details]
(2008) 'Cdc7 kinase inhibitors: pyrrolopyridinones as potential antitumor agents. 1. Synthesis and structure-activity relationships'
Vanotti, E. and Amici, R. and Bargiotti, A. and Berthelsen, J. and Bosotti, R. and Ciavolella, A. and Cirla, A. and Cristiani, C. and D'Alessio, R. and Forte, B. and Isacchi, A. and Martina, K. and Menichincheri, M. and Molinari, A. and Montagnoli, A. and Orsini, P. and Pillan, A. and Roletto, F. and Scolaro, A. and Tibolla, M. and Valsasina, B. and Varasi, M. and Volpi, D. and Santocanale, C. (2008) 'Cdc7 kinase inhibitors: pyrrolopyridinones as potential antitumor agents. 1. Synthesis and structure-activity relationships'. J Med Chem, 51 (3):487-501 [DOI] [Details]
(2008) 'A Cdc7 kinase inhibitor restricts initiation of DNA replication and has antitumor activity'
Montagnoli, A. and Valsasina, B. and Croci, V. and Menichincheri, M. and Rainoldi, S. and Marchesi, V. and Tibolla, M. and Tenca, P. and Brotherton, D. and Albanese, C. and Patton, V. and Alzani, R. and Ciavolella, A. and Sola, F. and Molinari, A. and Volpi, D. and Avanzi, N. and Fiorentini, F. and Cattoni, M. and Healy, S. and Ballinari, D. and Pesenti, E. and Isacchi, A. and Moll, J. and Bensimon, A. and Vanotti, E. and Santocanale, C. (2008) 'A Cdc7 kinase inhibitor restricts initiation of DNA replication and has antitumor activity'. Nat Chem Biol, 4 (6):357-65 [DOI] [Details]
(2007) 'Cdc7 is an active kinase in human cancer cells undergoing replication stress'
Tenca, P. and Brotherton, D. and Montagnoli, A. and Rainoldi, S. and Albanese, C. and Santocanale, C. (2007) 'Cdc7 is an active kinase in human cancer cells undergoing replication stress'. J Biol Chem, 282 (1):208-15 [DOI] [Details]
(2006) 'Identification of Mcm2 phosphorylation sites by S-phase-regulating kinases'
Montagnoli, A. and Valsasina, B. and Brotherton, D. and Troiani, S. and Rainoldi, S. and Tenca, P. and Molinari, A. and Santocanale, C. (2006) 'Identification of Mcm2 phosphorylation sites by S-phase-regulating kinases'. J Biol Chem, 281 (15):10281-90 [DOI] [Details]
(2002) 'Drf1, a novel regulatory subunit for human Cdc7 kinase'
Montagnoli, A. and Bosotti, R. and Villa, F. and Rialland, M. and Brotherton, D. and Mercurio, C. and Berthelsen, J. and Santocanale, C. (2002) 'Drf1, a novel regulatory subunit for human Cdc7 kinase'. EMBO J, 21 (12):3171-81 [DOI] [Details]
(2000) 'Dbf4p, an essential S phase-promoting factor, is targeted for degradation by the anaphase-promoting complex'
Ferreira, M. F. and Santocanale, C. and Drury, L. S. and Diffley, J. F. (2000) 'Dbf4p, an essential S phase-promoting factor, is targeted for degradation by the anaphase-promoting complex'. Mol Cell Biol, 20 (1):242-8 [DOI] [Details]
(2000) 'Coping with and recovering from hydroxyurea-induced replication fork arrest in budding yeast'
Diffley, J. F. and Bousset, K. and Labib, K. and Noton, E. A. and Santocanale, C. and Tercero, J. A. (2000) 'Coping with and recovering from hydroxyurea-induced replication fork arrest in budding yeast'. Cold Spring Harb Symp Quant Biol, 65 :333-42 [DOI] [Details]
(1999) 'Activation of dormant origins of DNA replication in budding yeast'
Santocanale, C. and Sharma, K. and Diffley, J. F. (1999) 'Activation of dormant origins of DNA replication in budding yeast'. Genes Dev, 13 (18):2360-4 [DOI] [Details]
(1998) 'A Mec1- and Rad53-dependent checkpoint controls late-firing origins of DNA replication'
Santocanale, C. and Diffley, J. F. (1998) 'A Mec1- and Rad53-dependent checkpoint controls late-firing origins of DNA replication'. Nature, 395 (6702):615-8 [DOI] [Details]
(1998) 'Evidence for a Cdc6p-independent mitotic resetting event involving DNA polymerase alpha'
Desdouets, C. and Santocanale, C. and Drury, L. S. and Perkins, G. and Foiani, M. and Plevani, P. and Diffley, J. F. (1998) 'Evidence for a Cdc6p-independent mitotic resetting event involving DNA polymerase alpha'. EMBO J, 17 (14):4139-46 [DOI] [Details]
(1997) 'Genomic footprinting of budding yeast replication origins during the cell cycle'
Santocanale, C. and Diffley, J. F. (1997) 'Genomic footprinting of budding yeast replication origins during the cell cycle'. Methods Enzymol, 283 :377-90 [DOI] [Details]
(1996) 'ORC- and Cdc6-dependent complexes at active and inactive chromosomal replication origins in Saccharomyces cerevisiae'
Santocanale, C. and Diffley, J. F. (1996) 'ORC- and Cdc6-dependent complexes at active and inactive chromosomal replication origins in Saccharomyces cerevisiae'. EMBO J, 15 (23):6671-9 [Details]
(1996) 'An essential role for the Cdc6 protein in forming the pre-replicative complexes of budding yeast'
Cocker, J. H. and Piatti, S. and Santocanale, C. and Nasmyth, K. and Diffley, J. F. (1996) 'An essential role for the Cdc6 protein in forming the pre-replicative complexes of budding yeast'. Nature, 379 (6561):180-2 [DOI] [Details]
(1995) 'Mutations in the gene encoding the 34 kDa subunit of yeast replication protein A cause defective S phase progression'
Santocanale, C. and Neecke, H. and Longhese, M. P. and Lucchini, G. and Plevani, P. (1995) 'Mutations in the gene encoding the 34 kDa subunit of yeast replication protein A cause defective S phase progression'. J Mol Biol, 254 (4):595-607 [DOI] [Details]
(1993) 'Purification and characterization of a new DNA polymerase from budding yeast Saccharomyces cerevisiae. A probable homolog of mammalian DNA polymerase beta'
Shimizu, K. and Santocanale, C. and Ropp, P. A. and Longhese, M. P. and Plevani, P. and Lucchini, G. and Sugino, A. (1993) 'Purification and characterization of a new DNA polymerase from budding yeast Saccharomyces cerevisiae. A probable homolog of mammalian DNA polymerase beta'. J Biol Chem, 268 (36):27148-53 [Details]
(1993) 'The isolated 48,000-dalton subunit of yeast DNA primase is sufficient for RNA primer synthesis'
Santocanale, C. and Foiani, M. and Lucchini, G. and Plevani, P. (1993) 'The isolated 48,000-dalton subunit of yeast DNA primase is sufficient for RNA primer synthesis'. J Biol Chem, 268 (2):1343-8 [Details]
(1992) 'Overproduction and functional analysis of DNA primase subunits from yeast and mouse'
Santocanale, C. and Locati, F. and Muzi Falconi, M. and Piseri, A. and Tseng, B. Y. and Lucchini, G. and Plevani, P. (1992) 'Overproduction and functional analysis of DNA primase subunits from yeast and mouse'. Gene, 113 (2):199-205 [DOI] [Details]
(1991) 'Mutations in conserved yeast DNA primase domains impair DNA replication in vivo'
Francesconi, S. and Longhese, M. P. and Piseri, A. and Santocanale, C. and Lucchini, G. and Plevani, P. (1991) 'Mutations in conserved yeast DNA primase domains impair DNA replication in vivo'. Proc Natl Acad Sci U S A, 88 (9):3877-81 [DOI] [Details]
(1989) 'A single essential gene, PRI2, encodes the large subunit of DNA primase in Saccharomyces cerevisiae'
Foiani, M. and Santocanale, C. and Plevani, P. and Lucchini, G. (1989) 'A single essential gene, PRI2, encodes the large subunit of DNA primase in Saccharomyces cerevisiae'. Mol Cell Biol, 9 (7):3081-7 [DOI] [Details]
(1988) 'The yeast DNA polymerase-primase complex: genes and proteins'
Plevani, P. and Foiani, M. and Muzi Falconi, M. and Pizzagalli, A. and Santocanale, C. and Francesconi, S. and Valsasnini, P. and Comedini, A. and Piatti, S. and Lucchini, G. (1988) 'The yeast DNA polymerase-primase complex: genes and proteins'. Biochim Biophys Acta, 951 (2-3):268-73 [DOI] [Details]

Other Journals

  Year Publication
(2023) 'DBF4, not DRF1, is the crucial regulator of CDC7 kinase at replication forks'
Anja Göder, Chrystelle Maric, Michael D. Rainey, Chiara Cazzaniga, Daniel Shamavu, Jean-Charles Cadoret, and Corrado Santocanale (2023) 'DBF4, not DRF1, is the crucial regulator of CDC7 kinase at replication forks' BIORXIV, Preprint . [Details]
(2023) 'CDC7 Inhibition Drives an Inflammatory Response and a p53-Dependent Senescent-Like State in Breast Epithelial Cells'
Chiara Cazzaniga, Anja Göder, Aisling Quinlan, Michael D. Rainey, Simone Coughlan, Stefanus Bernard and Corrado Santocanale (2023) 'CDC7 Inhibition Drives an Inflammatory Response and a p53-Dependent Senescent-Like State in Breast Epithelial Cells' Sneak Peak - Cell Press, preprint . [Details]

Employment

  Employer Position From / To
University of Galway Professor of Molecular Medicine 01-SEP-07 /
Pharmacia/Pfizer/NMS Senior research scientist 01-JUL-99 / 31-JUL-07
ICRF Postdoctoral researcher 01-OCT-94 / 30-JUN-99

Education

  Year Institution Qualification Subject
1993 University of Milan PhD Molecular Biology
1988 University of Milan MSc Biology

Languages

  Language
Italian
French
English

Teaching Interests

Biomolecules, DNA replication, Cell cycle, DNA repair, Cancer biology, Drug discovery and development

Recent Postgraduate Students

  Graduation Name Degree Primary Supervisor
2023 Clarisse Reyes PhD
2022 Chiara Cazzaniga PhD Y
2022 Aisling Quinlan PhD Y
2020 Rachel O'Dea PhD Y
2017 Jake McAulliffe Msc Y
2016 Edel McGarry PhD Y
2015 Kevin Wu PhD Y
2015 Ashton Scully Msc Y
2014 Mark Coyne PhD Y
2016 Aisling O'Connor PhD Y
2107 Huong Quach Thi Thu PhD Y
2016 Adam Brown Msc Y
2012 Anna Kliszczak PhD Y
2012 Raffaella D'Auria Msc Y

Current Postgraduate Students (Research)

  Student Degree Type Type
Conor Lowndes Doctorate - Structured PhD (Science) Co-supervisor (1)
Daniel Shamavu Doctorate - Structured PhD (Science) Supervisor
Stefanus Bernard Doctorate - Structured PhD (Science) Supervisor

Modules Taught

  Term/Year Module Title Module Code Subject / Desc
1st/2324 BO201
1st/2324 BI319
2nd/2324 BI453
2nd/2324 BI429
1st/2324 BI447
1st/2324 MD206
2nd/2324 BM202