Rapid Bioanalytical Methods

Research Goals Current Projects Completed Projects Results Publications Collaborations & Funding

Research Goals

The primary goal of our bioanalytical research is concerned with developing rapid, quantitative spectroscopic based techniques for the non-contact, non-destructive analysis of the complex materials (e.g. raw materials, cell culture media, bioprocess broths) encountered in biological based pharmaceutical manufacturing.

The value of the methods that we are developing lies in the fact that they are inexpensive and suitable for screening all the materials used in a BioPharmaceutical manufacturing process.

These methods are designed to be very rapid, require no expensive training or consumables and are also perfectly suited to use in aseptic methods of production and harsh environments.

To date (Nov. 2024) we have mostly focused on the quantitative analysis of cell culture media, the raw materials used for medias, and bioprocess broths.  In 2024 a new project started which is looking at downstream processing of monoclonal antibodies and large biological systems. 

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Current Projects

We are currently undertaking a wide variety of individual research projects.  In general, the work involves the use of spectroscopic techniques like Atomic Emission Spectroscopy, Raman, FT-IR, FT-NIR, SERS, UV-VIS, DLS, and multi-dimensional Fluorescence for the quantitative analysis of complex mixtures.  The spectroscopic data is then analyzed using advanced chemometric methods which are developed in-house. In the final stages of the research we also engage in the technical transfer activities required e.g. preparation of Standard Operating Procedures, Validation experiments, etc.  Details will be posted as and when they are accepted for publication, however much of what we do is covered by confidentiality agreements.

Downstream Protein Analysis - Polarized Emission Spectroscopy (DPA-PES), 2024-29:

MNC 2 (2024-26): 

This project is developing novel analytical methods for large macromolecules.  This research involves the use of Fluorescence spectroscopy.

Horiba  (2021-present):

We are collaborating with Horiba on the use of fluorescence EEM measurements for biopharmaceutical manufacturing applications using their Aqualog CCD based fluorescence spectrometer.  We currently have two Aqualogs installed in the lab, configured for pEEM measurements in cuvettes and in flow.  

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Completed Projects:

CBAS (Phase 1, BMS), 2005-09:

A team of ~10 researchers was based in the Nanoscale Biophotonics Laboratory on this project. The CBAS project was a collaboration with Bristol-Myers Squibb and Dublin City University and is funded by the IDA and BMS.
Press releases on the project can be found on the Galway Advertiser, and IDA website.
A discussion piece is also available on: Pharma Manufacturing.Com.
This project is now complete and we published multiple outcomes from this research.

Merck, 2011-12.

We undertook a collaboration with Merck on the analysis of cell culture media and the development of new analytical methods.This research involved the use of Raman and Fluorescence spectroscopies.

Janssen Biologics (2010-11, 2013-15):

The first industrial collaboration with the Janssen Pharmaceutical Companies of Johnson & Johnson began on the 01/09/10.  The initial project, funded by J&J  and NIBRT developed new methods for characterizing cell culture media. Subsequent follow-on projects expanded on these initial studies and we were engaged in a variety of studies using Raman and Fluorescence spectroscopies. There were two postdoctoral researchers engaged full-time on this project.

GSK (2017-19):

This project focussed on developing robust analytical methods for assessing cell culture media quality.  Soem of the work using Size Exclusion Chromatography has now been published here:  DOI: 10.1016/j.jbiotec.2023.09.001

MNC (2017-19):

We had a project with an Irish based multi-national.  This project  focussed on developing robust analytical methods for assessing cell culture media quality.

Agilent (2013-22):

We collaborated with Agilent on the development of a new fluorescence based analytical platform for the analysis of complex biogenic liquids.

Kaiser Optical Systems Inc. (2011-20):

We collaboratedwith KOSI as part of the Raman centre of excellence Programme. This has resulted in KOSI providing a variety of Raman workstations for use in both small and large molecule API research studies. 

Advanced Analytics for Biopharmaceutical Manufacturing, AA-BTM (2015-22):

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Selected Results

We will upload data when appropriate. We expect to be able to publish a range of manuscripts detailing our work over the past 5 years, once reviewed by our industrial partners. In the meantime the published manuscripts should provide an overview of what has been done to date.  

Cell culture media: 

Mammalian cell culture is increasingly being used for the manufacture of recombinant protein based therapeutics. One of the key components of the cell culture manufacturing process is the area of cell culture media (CCM).  The fields of CCM formulation, storage, feeding, and analysis is a critical area of bio-analytical science which is complicated by the fact the CCMs are very complex and sensitive materials, particularly when in liquid form.   CCMs can consist of a wide variety of diverse components: sugars, amino acids, proteins, vitamins, inorganic salts and other nutrients. However the rapid, online, non-destructive, and quantitative analysis of CCMs is currently and under-developed area, but there is an urgent need for these types of methods. Rapid and/or real-time monitoring of CCM composition would provide a tool to improve process control and product yield. To optimize the product yield, the CCM composition and feeding rates must be tightly controlled.

Motivated by the need for rapid, non-contact, non-destructive and inexpensive methods of analysis for these complex CCM mixtures, we are exploring the application of Raman and Fluorescence spectroscopy coupled with mathematical analysis for robust, reliable, and rapid CCM characterization.

Jan. 11: Our work on rapid cell culture media analysis was featured in the January issue of Genetic Engineering & Biotechnology News.  The article can be accessed here. 

Bioprocess broths:

The analysis of complex cell culture process broths is challenging, time-consuming, and expensive due to the fact that they are complex mixtures  and consist of a vast variety of components, e.g., inorganic salts, sugars, amino acids, proteins, vitamins, and other nutrients. This thus requires the development of holistic and inexpensive methods that can elucidate compositional information from these complex mixtures for diagnostic and process control purposes. Here we have used a range of spectroscopic methods coupled with advanced Chemometrics to develop a suite of characterization tools which are all suited to online process control. We have published two main studies in collaboration with Bristol-Myers Squibb on the use of fluorescence EEM and Raman spectroscopy respectively.  In both studies it was possible to use the spectral data to predict process performance (in terms of final product yield) at an early stage in the bioprocess.

BioPharma raw materials:

A lot of the raw materials or third party supplied media components are very complex mixtures (solid or liquid) and they are challenging from an ID and quality assessment point of view. We have developed a range of very inexpensive ID and quality tools for use in both the QC lab and in the storeroom.

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Publications and Conference Presentations

More details, posters, and manuscripts will be uploaded as they become available.  The works listed below are a combination of CBAS, other industry media analysis projects, and relevant non-industry research undertaken in our lab.

Publications (updated 19-09-2023): 

1. Size Exclusion Chromatography for Screening Yeastolate used in cell culture media.  M. Kyne, A.-L. de Faria e Silva, B. Vickroy, and A.G. Ryder. 376, 1-10, Journal of Biotechnology, (2023).  DOI: 10.1016/j.jbiotec.2023.09.001
2. Development of a rapid Polarized Total Synchronous Fluorescence Spectroscopy (pTSFS) method for protein quantification in a model bioreactor broth.  B.O. Boateng, S. Elcoroaristizabal, and A.G. Ryder, Biotechnology and Bioengineering,  118(5), 1805-1817, (2021).  DOI: 10.1002/bit.27694 [Open access]
3. Cell Culture Media Analysis using Rapid Spectroscopic Methods.  A.G. Ryder.  Current Opinion in Chemical Engineering, 22, 11-17, (2018).  [peer reviewed review]  DOI:  10.1016/j.coche.2018.08.008
4. Calibration, Standardization, and Quantitative Analysis of Multidimensional Fluorescence (MDF) Measurements on Complex Mixtures (IUPAC Technical Report).  A.G. Ryder, C.A. Stedmon, N. Harrit, and R. Bro.  Pure and Applied Chemistry , 89(12), 1849-1870, (2017).    DOI: 10.1515/pac-2017-0610 
5. Raman Spectroscopy in Biopharmaceutical Manufacturing:  a critical review.  K. Buckley and A.G. Ryder.  Applied Spectroscopy, 71(6), 1085-1116, (2017).  Focal point article (peer reviewed).   DOI: 10.1177/0003702817703270  
   
6. Anisotropy Resolved Multidimensional Emission Spectroscopy (ARMES): a new tool for protein analysis.  R.C. Groza, B. Li, and A.G. Ryder.   Analytica Chimica Acta, 886, 133-142, (2015).   DOI: 10.1016/j.aca.2015.06.011.
7. Monitoring Cell Culture Media Degradation using SERS Spectroscopy.  A. Calvet and A.G. Ryder.   Analytica Chimica Acta, 840, 58-67, (2014).   DOI:  10.1016/j.aca.2014.06.021 
8. A Fluorescence Anisotropy Method for Protein Concentration Monitoring in Complex Cell Culture Media.  R. C. Groza, A. Calvet, and A. G. Ryder. Analytica Chimica Acta, 821, 54-61, (2014).
   DOI: 10.1016/j.aca.2014.03.007
9. Comprehensive Quantitative Bioprocess Monitoring using Fluorescence spectroscopy and chemometrics.  B. Li, M. Shanahan, A. Calvet, K.J. Leister, and A. G. Ryder.  Analyst, 139(7), 1661-1671, (2014).   DOI:  10.1039/C4AN00007B.
10. A rapid fluorescence based method for the quantitative analysis of cell culture media photo-degradation. A. Calvet, B. Li, and A.G. Ryder.  Analytica Chimica Acta, 807, 111-119, (2014).
    DOI: 10.1016/j.aca.2013.11.028
11. Performance Monitoring of a Mammalian Cell Based Bioprocess using Raman spectroscopy.  B. Li, B.H. Ray, K.J. Leister, and A.G. Ryder.  Analytica Chimica Acta, 796, 84-91, (2013).
    DOI:  10.1016/j.aca.2013.07.058 
12. Rapid Quantification of Tryptophan and Tyrosine in Chemically Defined Cell Culture Media using Fluorescence Spectroscopy. A. Calvet, B. Li, and A. G. Ryder.  Journal of Pharmaceutical and Biomedical Analysis, 71, 89-98, (2012). DOI: 10.1016/j.jpba.2012.08.002
13. Using Surface-Enhanced Raman Scattering (SERS) and Fluorescence Spectroscopy for Screening Yeast Extracts, A Complex Component of Cell Culture Media. B. Li, N.M.S. Sirimuthu, B. Ray, and A.G. Ryder,   Journal of Raman Spectroscopy, 43(8), 1074-1082, (2012).   DOI:  10.1002/jrs.3141
14. Fluorescence EEM Spectroscopy for Rapid Identification and Quality Evaluation of Cell Culture Media Components.  B. Li, P.W. Ryan, M. Shanahan, K.J. Leister, and A.G. Ryder. Applied Spectroscopy, 65(11), 1240-1249, (2011).   DOI:  10.1366/11-06383.
15. Rapid, low-cost, quantitative characterization of cell culture media using a combination of spectroscopic and chemometric methods.  A.G. Ryder, B. Li, and K.J. Leister.  International Drug Discovery, Oct./Nov. issue, 18-21, (2010).
16. Rapid Characterization and Quality Control of Complex Cell Culture Media Solutions using Raman Spectroscopy and Chemometrics. B. Li, P.W. Ryan, B.H. Ray, K.J. Leister, N.M.S. Sirimuthu, and A.G. Ryder.  Biotechnology and Bioengineering, 107(2), 290-301, (2010).   DOI: 10.1002/bit.22813 .
17. Prediction of Cell Culture Media performance using Fluorescence Spectroscopy.  P.W. Ryan, B. Li, M. Shanahan, K.J. Leister, and A.G. Ryder, Analytical Chemistry, 82(4), 1311-1317, (2010).
    DOI: 10.1021/ac902337c 
18. A Stainless Steel Multi-Well Plate (SS-MWP) for High Throughput Raman Analysis of Dilute Solutions. A.G. Ryder, J. de Vincentis, B. Li, P.W. Ryan, N.M.S. Sirimuthu, and K.J. Leister.   Journal of Raman Spectroscopy, 41(10), 1266-1275, (2010).   DOI: 10.1002/jrs.2586.

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Relevant Invited Presentations (as of 15/04/2024): 

1. Complexity in Raman Spectroscopy: the curse of the N’s with samples from biopharmaceutical manufacturing.  SciX 2022, Covington KY, USA, 2-7 October, 2022.
2. The application of multi-dimensional fluorescence spectroscopy, anisotropy, and chemometrics as a process-wide, analytical technology platform for Biopharmaceuticals.  EuChemS at ECC8 Chemistry Congress, Lisbon Portugal, 28 August - 1 September, 2022. 
3. Using Intrinsic Fluorescence Emission for Analysis of Cell Culture Media and Protein-Based Samples, Bioprocessing Summit Europe, The Crowne Plaza, Barcelona (delivered online), Spain, 21-23, July, 2020.
4. Combining multi-dimensional fluorescence spectroscopy with chemometric data analysis for routine and effective raw material & cell culture media analysis. Optimizing Cell Culture Technology at The Bioprocessing Summit, Seaport World Trade Center, Boston, Mass., USA, 12-13 Aug., 2019.
5. Bioprocess monitoring and protein quality assessment using polarized intrinsic fluorescence spectroscopy in multi-dimensional modes:  a new measurement methodology. Analyzing / Monitoring Processes to Ensure Quality at The Bioprocessing Summit, Seaport World Trade Center, Boston, Mass., USA, 14-15 Aug., 2019.
6. Cell culture media powder (CCMp) analysis by vibrational spectroscopy: why Raman, NIR, and FT-IR can be problematical.  A.G. Ryder.  IFPAC-2019,  Bethesda, MD, USA, 3-6 Mar. 2019.
7. Anisotropy Resolved Multi-dimensional Emission Spectroscopy (ARMES) for the analysis of proteins in solution: a new analytical paradigm,  A.G. Ryder, BioProduction Congress, Dublin, 9-10 Oct. 2018 (invited).
8. Using fluorescence spectroscopy for the analysis of raw material and cell culture media variability. A.G. Ryder, BioProduction Congress, Dublin, 9-10 Oct. 2018 (invited).
9. Using multi-dimensional fluorescence spectroscopy measurements in Biopharmaceutical Manufacturing: the need for better multidimensional standards,”  A.G. Ryder, 15th International Symposium on Biological and Environmental Reference Materials (BERM 15, Worksop on Optical Spectroscopy), BAM Berlin, Germany, 24-26 Sept., 2018 (invited).
10. Complexity, the Bugbear Preventing Synergy Between Real Time Analytics in Small vs. Large Molecules. A.G. Ryder.  IFPAC-2018,  Bethesda, MD, USA, 11-14 Feb. 2018 (invited).
11. Developing multi-dimensional fluorescence spectroscopies as a process-wide. platform analytical technology for Biopharmaceutical analysis. A.G. Ryder, EuPAT8, Cork, Ireland, 3-4 Oct. 2016 (invited).
12. The use of multi-dimensional fluorescence spectroscopy for the quantitative analysis of liquid media:  from hydrolysates to protein solutions.  Eastern Analytical Symposium, Somerset, NJ, USA, 16-18 Nov., 2015 (invited).
13. Comprehensive, Quantitative Bioprocess Productivity Monitoring Using Fluorescence EEM Spectroscopy and Chemometrics, PEGS Europe:  Protein & Antibody Engineering Summit, Lisbon Portugal, 2-6 Nov. 2015 (invited).
14. Advances in the use of multi-dimensional fluorescence spectroscopies for the quantitative analysis of cell culture media and proteins:  new tools for process and product analytics.  Animal Cell Technology Industrial Platform meeting, Lisbon Portugal, 6 - 8 May, 2015. 
15. T1: Comprehensive, quantitative bioprocess monitoring using fluorescence spectroscopy. & T2: Fast, easy, and reliable methods for quantifying cell culture media variance and stability:  Or…how to taste your super soup…. The Bioprocessing Summit , Renaissance Boston Waterfront Hotel, Boston, Mass., USA, Aug. 18-22, 2014.
16. Quantitative monitoring raw material and cell culture media quality using fast fluorescence based analytical methods.,  3rd annual Biopharmaceutical Raw Materials: Qualification, Analysis, & supply chain management, Cologne, Germany, 24-25 June, 2014.
17. A Comprehensive Analytical Platform for Biopharmaceutical Manufacturing: photonics & vegemite.  Biomanufacturing Innovation Group Meeting, Improving Process Knowledge, NIBRT, Dublin, 27th Feb. 2014.
18. Quantitative performance monitoring of a mammalian cell based bioprocess using Raman spectroscopy.  Bioproduction 2013, Dublin, 22-23, Oct. 2013.
    Raman spectroscopy in biopharmaceutical manufacturing:  SciX 2013, Milwaukee, WI, USA, 29 Sept. – 4 Oct., 2013.
19. Quantitative Analysis of Complex Liquids using Multi-dimensional Fluorescence Spectroscopy: From Oil to Vegemite, Applied Industrial Optics 2013,  Arlington Virginia, USA, 23-27 June, 2013.
20. Fluorescence Excitation-Emission matrix spectroscopy in BioPharma,  Irish Chemometrics Meeting , Teasgasc Food Research Centre, Ashtown, Dublin, 06 Dec. 2012. 
21. Industry-Academia case study in Biopharmaceutical analysis, ISPE Ireland Affiliate Event , NIBRT, UCD, 08 Nov. 2012.
22. Quantitative analysis of cell culture media using multi-dimensional fluorescence spectroscopy, A.G. Ryder,  Biopharmaceutical Raw Materials: Qualification, Analysis, & supply chain management, Cologne, Germany, 27-28 June, 2012..
23. Quantitative Analysis of Cell Culture Media using Raman Spectroscopy. A. Ryder,  26th  International Forum and Exhibition Process Analytical Technology,  Baltimore, MD, USA, 22-25 Jan. 2012.
24. Rapid, Quantitative analytical methods for hydrosylates using in cell culture media:  a spectroscopic approach.  Alan Ryder,  Process2Product, Translating Process Knowledge into Quality Biological Products, Washington D.C., USA, 4-6 October 2011.
25. Rapid, Low-Cost, Quantitative Characterization of Cell Culture Media Using a Combination of Spectroscopic and Chemometric Methods.  Alan Ryder,  Process2Product, Translating Process Knowledge into Quality Biological Products, Bethesda, Maryland, USA, 21-22 Oct., 2010.

Relevant Conference Poster & Oral Presentations (as of 15-04-2024): 

1. Assessing Lot-to-Lot Elemental Variability in Cell Culture Media using Microwave Plasma Atomic Emission Spectroscopy. S. Grant & A.G. Ryder. SCIX2023, Sparks Nevada, USA, October 7-13, 2023. [Oral presentation]
2. Protein Quantification in a model bioreactor broth using polarized Total Synchronous Fluorescence Spectroscopy (pTSFS).  B. Boateng,* S. Elcoroaristizabal, A.G. Ryder.  IFPAC-2019,  Bethesda, MD, USA, 3-6 Mar.  2019 (oral pres.). 
3. Assessing IgG PEGylation reactions and products using polarized Excitation Emission Matrix (pEEM) spectroscopy.  A.-L. de Faria e Silva,* S. Elcoroaristizabal, A.G. Ryder.  IFPAC-2019,  Bethesda, MD, USA, 3-6 Mar. 2019 (oral pres.).
4. Assessing IgG quality using polarized Excitation Emission Matrix (pEEM) spectroscopy.  A.-L. de Faria e Silva,* S. Elcoroaristizabal, A.G. Ryder.  IFPAC-2019,  Bethesda, MD, USA, 3-6 Mar. 2019.  [2nd prize in poster competition]. 
5. Effect of signal to noise ratio on polarized Total Synchronous Fluorescence Spectroscopy (pTSFS) measurements used for bioprocess analysis.  B. Boateng,* S. Elcoroaristizabal, A.G. Ryder.  IFPAC-2019,  Bethesda, MD, USA, 3-6 Mar. 2019. [Poster]
6. Assessing freeze-thaw effects in IgG using Anisotropy Resolved Multi-Dimensional Emission Spectroscopy (ARMES).  M. Steiner-Browne,* S. Elcoroaristizabal, Y. Casamayou-Boucau, and A.G. Ryder.  BioPharma Ambition 2018 , Dublin Castle, 21-11 Feb. 2018.  [Winning poster].
7. A new rapid analytical methodology for soluble insulin aggregate analysis.Y. Casamayou-Boucau* and A.G. Ryder.  BioPharma Ambition 2018, Dublin Castle, 21-11 Feb. 2018.
8. Characterizing PEGylation reactions using multidimensional fluorescence (MDF) spectroscopy.  A.L. de Faria*, S. Elcoroaristizabal, Y. Casamayou-Boucau, and A.G. Ryder.  BioPharma Ambition 2018, Dublin Castle, 21-11 Feb. 2018.
9. “Monitoring Structural Stability and Aggregation of Immunoglobulin G (IgG) Under Thermal Stress using Anisotropy Resolved Multidimensional Emission Spectroscopy (ARMES).”  M. Steiner,* S. Elcoroaristizabal, Y. Casamayou-Boucau, and A.G. Ryder.  15th Conference on Methods and Applications of Fluorescence: Spectroscopy, Imaging and Probes, MAF15, Bruges, Belgium, 10-13 Sept. 2017.
10. “Anisotropy resolved multidimensional emission spectroscopy (ARMES) for discrimination of mixed serum albumin solutions.”  S. Elcoroaristizabal and A.G. Ryder.  15th Conference on Methods and Applications of Fluorescence: Spectroscopy, Imaging and Probes, MAF15, Bruges, Belgium, 10-13 Sept. 2017.
11. “Characterizing IgG conjugation products using Anisotropy Resolved Multi-Dimensional Spectroscopy (ARMES).”  A.L. de Faria,* S. Elcoroaristizabal, Y. Casamayou-Boucau, and A.G. Ryder. 1 15th Conference on Methods and Applications of Fluorescence: Spectroscopy, Imaging and Probes, MAF15, Bruges, Belgium, 10-13 Sept. 2017.
12. Anisotropy Resolved Multi-dimensional Emission Spectroscopy (ARMES): A new tool for protein structure analysis, R.C. Groza, B. Li, and A.G. Ryder.  14th Conference on Methods and Applications of Fluorescence: Spectroscopy, Imaging and Probes, Würzburg, Germany, 13–16 Sept., 2015.
13. Fluorescence Spectroscopy Monitoring of Mammalian Cell Based Recombinant Protein Manufacturing Process Performance, 13th Conference on Methods and Applications of Fluorescence: Spectroscopy, Imaging and Probes, Genoa, Italy, 8 – 11 Sept., 2013.
14. Combining Multidimensional Fluorescence Spectroscopy and Chemometrics for Industrial Biopharmaceutical Applications, A.G. Ryder, B. Li, A. Calvet, C. Morris, M. Shanahan, B. Kissane, 13th Conference on Methods and Applications of Fluorescence: Spectroscopy, Imaging and Probes, Genoa, Italy, 8 – 11 Sept., 2013.
15. Analysis of cell culture media by multi-dimensional fluorescence anisotropy.  R. C. Groza and A. G. Ryder, 13th Conference on Methods and Applications of Fluorescence: Spectroscopy, Imaging and Probes, Genoa, Italy, 8 – 11 Sept., 2013.
16. The use of multi-dimensional fluorescence for the monitoring of cell culture media degradation, A. Calvet, C. Morris and A. G. Ryder. 13th Conference on Methods and Applications of Fluorescence: Spectroscopy, Imaging and Probes, Genoa, Italy, 8 – 11 Sept., 2013.
17. Quantification of amino acids and vitamins in cell culture media using Excitation-Emission Matrix fluorescence spectroscopy and chemometrics.  A. Calvet and A.G. Ryder, 1 2th Conference on Methods and Applications of Fluorescence: Spectroscopy, Imaging and Probes, Strasbourg, France, 11-14 Sept., 2011.
18. Prediction of bioprocess performance by multidimensional fluorescence spectroscopy analysis of bioreactor broths.  B. Li and A.G. Ryder, 12th Conference on Methods and Applications of Fluorescence: Spectroscopy, Imaging and Probes, Strasbourg, France, 11-14 Sept., 2011.
19. Quantitative analysis of yeastolate and eRDF in model cell culture media using fluorescence spectroscopy.  B. Kissane, B. Li, and A.G. Ryder, 12th Conference on Methods and Applications of Fluorescence: Spectroscopy, Imaging and Probes, Strasbourg, France, 11-14 Sept., 2011. 
20. Quantitative Analysis of Yeastolate and eRDF in aqueous mixtures by Surface Enhanced Raman Scattering (SERS).  B. Kissane, B. Li, N.M.S. Sirimuthu, and A.G. Ryder. RSC Analytical Research Forum 2011, University of Manchester, UK, 25 - 27 July 2011.
21. 2D fluorometry to monitor CHO cell based bioprocesses used in biopharmaceutical manufacturing.  B. Li and A. G. Ryder. RSC Analytical Research Forum 2011, University of Manchester, UK, 25 - 27 July 2011.
22. Component Tracking of Complex Aqueous Cell Culture Media Using Multidimensional Fluorescence and Chemometric Methods.  B. Li, A.G. Ryder, P.W. Ryan, and K.J. Leister.  11th Conference on Methods and Applications of Fluorescence: Spectroscopy, Imaging and Probes, Budapest, Hungary, 6-9 Sept., 2009.
23. Determination of glucose concentration in cell culture media by Raman spectroscopy and multivariate calibration. B. Kissane, A.G. Ryder, & B. Li, 42nd IUPAC Congress, Glasgow, 2-7 Aug. 2009.
24. Characterisation of hydroxyapatite and dicalcium phosphate using X-Ray Fluorescence Spectrometry, N. Walshe, A.G. Ryder, C. O'Kane, & A.R. Boyd, 42nd IUPAC Congress, Glasgow, 2-7 Aug. 2009.
25. Process monitoring of a complex aqueous cell culture broth by multidimensional fluorescence and chemometric methods. B. Li, A. G. Ryder, P.W. Ryan, & K.J. Leister, 42nd IUPAC Congress, Glasgow, 2-7 Aug. 2009. 
26. A strategy for rapid Raman characterisation and quality control of cell culture media components. P.W. Ryan, B. Li, A.G. Ryder, K.J. Leister, B.H. Ray, & N.M.S. Sirimuthu, 42nd IUPAC Congress, Glasgow, 2-7 Aug. 2009.
27. Surface enhanced Raman spectroscopy (SERS): a powerful tool to monitor the degradation of complex raw materials used for Biopharmaceutical Manufacturing. A.G. Ryder, N.M.S. Sirimuthu, and B. Li. International Conference on Trends in Bioanalytical Sciences and Biosensors ( ICTBSB-2009), Dublin, 26-27 Jan., 2009.
28. Methods for the Rapid Identification of Cell Culture Media Components. P.W. Ryan, B. Li, A.G. Ryder, K.J. Leister, and N.M.S. Sirimuthu. International Conference on Trends in Bioanalytical Sciences and Biosensors ( ICTBSB-2009), Dublin, 26-27 Jan., 2009.
29. Multidimensional fluorescence analysis of complex aqueous cell culture media by chemometric methods.  B. Li, A.G. Ryder, P.W. Ryan, and K.J. Leister. International Conference on Trends in Bioanalytical Sciences and Biosensors ( ICTBSB-2009), Dublin, 26-27 Jan., 2009.
30. Quantitative Analysis of Yeastolate and eRDF in aqueous mixtures by Surface Enhanced Raman spectroscopy (SERS).  B. Kissane, A.G. Ryder, B. Li, and N.M.S. Sirimuthu. International Conference on Trends in Bioanalytical Sciences and Biosensors ( ICTBSB-2009), Dublin, 26-27 Jan., 2009.
31. Eliminating the water signal from Raman spectra of aqueous samples. R. Rajko, A.G. Ryder, B. Kissane, and B. Li.   Metabolomics Society 3 ^rd Annual Conference, Manchester, UK, 11-14 June 2007.
32. A novel similarity index for quality assessment of complex aqueous media by multidimensional fluorescence spectroscopy.  B. Li,  R. Rajko, B. Kissane, and A.G. Ryder.  Metabolomics Society 3 ^rd Annual Conference, Manchester, UK, 11-14 June, 2007.

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Collaborations & funding

We are happy to work with industrial partners in Europe and US who are interested in this type of spectroscopic approach to analysing complex biotechnology processes. 

For Bio-pharmaceutical companies based in Ireland there are a variety of support mechanisms:

• Enterprise Ireland offer a range of supports to leverage additional support for Industry-Academia partnerships.
• The Irish Research Council also offer the possibility of jointly funded PhD scholarships and postdoctoral posts under the Enterprise Partnership Scheme.
• SFI also  have a Technology and Innovation Development Award (TIDA) which can be used to support short 1 year, research projects. 

‌

Sheephaven Bay, Muckish Mountain...and Bloody Foreland in the distance.

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Contact:

Prof. Alan G. Ryder
Nanoscale Biophotonics Laboratory, Room 213, University of Galway, University Road, Galway, H91 TK33, Ireland.
Tel: +353 (0)91 492943 or ext. 2943 (internal)   E-mail: alan.ryder-AT-universityofgalway.ie

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