Biological and Chemical Sciences

Dr Andrew Flaus

BSc, PhD.

Biography

2015-present: Vice Dean for Internationalisation in College of Science and Engineering, NUI Galway
2009-2015: BSc Biotechnology programme director, NUI Galway
2006 to present: Lecturer, Department of Biochemistry and BSc Biotechnology programme, NUI Galway.
1999-2006: Postdoc with Dr Tom Owen-Hughes, Division of Gene Regulation and Expression, School of Life Sciences, University of Dundee, Scotland
1996-1998: Postdoc with Prof Tim Richmond, Institut f. Molekularbiologie u. Biophysik, ETH (Swiss Federal Inst of Technology), Zurich, Switzerland
1990-1995: PhD student with Prof Tim Richmond, Institut f. Molekularbiologie u. Biophysik, ETH (Swiss Federal Inst of Technology), Zurich, Switzerland
1986-1990: BSc (Hons) in Biochemistry and Molecular Genetics, Department of Chemistry and Biochemistry and Department of Genetics, Massey University, Palmerston North, New Zealand (honours research with Drs Gill Norris and Chris Moore)

Research Interests

Dynamic structure and function of nucleosomes, the basic building blocks of chromosomes. Mechanism of ATP-dependent chromatin remodelling enzymes. Biochemical techniques for probing protein-DNA interactions. Bioinformatic analysis of chromatin components. Protein stucture/function.

Peer Reviewed Journals

	Year	Publication
	(2019)	'Functional transcription promoters at DNA double-strand breaks mediate RNA-driven phase separation of damage-response factors' Pessina, F;Giavazzi, F;Yin, YD;Gioia, U;Vitelli, V;Galbiati, A;Barozzi, S;Garre, M;Oldani, A;Flaus, A;Cerbino, R;Parazzoli, D;Rothenberg, E;di Fagagna, FD (2019) 'Functional transcription promoters at DNA double-strand breaks mediate RNA-driven phase separation of damage-response factors'. Nature Cell Biology, 21 [DOI] [Details]
	(2010)	'Structure and Function of Histone H2AX' Pinto, DMS;Flaus, A (2010) 'Structure and Function of Histone H2AX'. Endotoxins: Structure, Function And Recognition, 50 :55-78 [DOI] [Details]
	(2011)	'Principles and practice of nucleosome positioning in vitro' Flaus, A (2011) 'Principles and practice of nucleosome positioning in vitro'. Frontiers In Life Science, 5 :5-27 [DOI] [Details]
	(2018)	'Viral proteins as a potential driver of histone depletion in dinoflagellates' Irwin, NAT,Martin, BJE,Young, BP,Browne, MJG,Flaus, A,Loewen, CJR,Keeling, PJ,Howe, LJ (2018) 'Viral proteins as a potential driver of histone depletion in dinoflagellates'. Nature Communications, 9 [DOI] [Details]
	(2015)	'A chromatin-independent role of Polycomb-like 1 to stabilize p53 and promote cellular quiescence' Brien, GL,Healy, E,Jerman, E,Conway, E,Fadda, E,O'Donovan, D,Krivtsov, AV,Rice, AM,Kearney, CJ,Flaus, A,McDade, SS,Martin, SJ,McLysaght, A,O'Connell, DJ,Armstrong, SA,Bracken, AP (2015) 'A chromatin-independent role of Polycomb-like 1 to stabilize p53 and promote cellular quiescence'. Genes & Development, 29 :2231-2243 [DOI] [Details]
	(2015)	'Histone H2AX Y142 phosphorylation is a low abundance modification' Hatimy, AA,Browne, MJG,Flaus, A,Sweet, SMM (2015) 'Histone H2AX Y142 phosphorylation is a low abundance modification'. Int J Mass Spectrom, 391 :139-145 [DOI] [Details]
	(2015)	'Life at the mesoscale: the self-organised cytoplasm and nucleoplasm' Sear, RP,Pagonabarraga, I,Flaus, A (2015) 'Life at the mesoscale: the self-organised cytoplasm and nucleoplasm'. Bmc Biophysics, 8 [DOI] [Details]
	(2011)	'Principles and practice of nucleosome positioning in vitro' Flaus, A (2011) 'Principles and practice of nucleosome positioning in vitro'. Frontiers In Life Science, 5 :5-27 [DOI] [Details]
	(2011)	'Mechanisms for ATP-dependent chromatin remodelling: The means to the end' Flaus, A., Owen-Hughes, T. (2011) 'Mechanisms for ATP-dependent chromatin remodelling: The means to the end'. FEBS Journal, 278 (19):3579-3595 [Details]
	(2010)	'Structure and function of histone H2AX' Pinto, DM, Flaus, A. (2010) 'Structure and function of histone H2AX'. Subcellular Biochemistry, 50 :55-78 [Details]
	(2009)	'Nucleosomes can invade DNA territories occupied by their neighbours' Engeholm M, de Jager M, Flaus A, Brenk R, van Noort J, Owen-Hughes T. (2009) 'Nucleosomes can invade DNA territories occupied by their neighbours'. Nature Structural & Molecular Biology, 16 (2):151-158 [Details]
	(2007)	'Histone tails and the H3 alphaN helix regulate nucleosome mobility and stability' Ferreira H, Somers J, Webster R, Flaus A, Owen-Hughes T. (2007) 'Histone tails and the H3 alphaN helix regulate nucleosome mobility and stability'. Mol Cell Biol, 11 :4037-4048 [Details]
	(2007)	'Histone modifications influence the action of Snf2 family remodelling enzymes by different mechanisms' Ferreira H, Flaus A, Owen-Hughes T. (2007) 'Histone modifications influence the action of Snf2 family remodelling enzymes by different mechanisms'. J Mol Biol, 374 (3):563-579 [Details]
	(2006)	'Chromatin modulation and the DNA damage response' Costelloe T, Fitzgerald J, Murphy NJ, Flaus, A & Lowndes, NF (2006) 'Chromatin modulation and the DNA damage response'. Exp Cell Res, 312 :2677-2686 [Details]
	(2006)	'Nucleosome dynamics' Stockdale C, Bruno M, Ferreira H, Garcia-Wilson E, Wiechens N, Engeholm M, Flaus A, Owen-Hughes T. (2006) 'Nucleosome dynamics'. Biochem Soc Symp, 73 :109-119 [Details]
	(2006)	'Identification of multiple distinct Snf2 subfamilies with conserved structural motifs' Flaus A, Martin DM, Barton GJ, Owen-Hughes T. (2006) 'Identification of multiple distinct Snf2 subfamilies with conserved structural motifs'. Nucleic Acids Res, 34 (10):2887-2905 [Details]
	(2006)	'Chromatin modulation and the DNA damage response' Costelloe T, Fitzgerald J, Murphy NJ, Flaus A, Lowndes NF (2006) 'Chromatin modulation and the DNA damage response'. Experimental Cell Research, 312 (14):2677-2686 [DOI] [Details]
	(2006)	'Analysis of nucleosome repositioning by yeast ISWI and Chd1 chromatin remodeling complexes' Stockdale C, Flaus A, Ferreira H, Owen-Hughes T. (2006) 'Analysis of nucleosome repositioning by yeast ISWI and Chd1 chromatin remodeling complexes'. J Biol Chem, 281 (24):16279-16288 [Details]
	(2006)	'Snf2 family ATPases and DExx box helicases: differences and unifying concepts from high-resolution crystal structures' Duerr, H, Flaus A, Owen-Hughes T, Hopfner KP. (2006) 'Snf2 family ATPases and DExx box helicases: differences and unifying concepts from high-resolution crystal structures'. Nucleic Acids Res, 34 (15):4160-4167 [Details]
	(2004)	'Site-specific attachment of reporter compounds to recombinant histones' Bruno M, Flaus A, Owen-Hughes T. (2004) 'Site-specific attachment of reporter compounds to recombinant histones'. Methods Enzymol, 375 :211-228 [Details]
	(2004)	'Mechanisms for ATP-dependent chromatin remodelling: farewell to the tuna-can octamer?' Flaus A, Owen-Hughes T. (2004) 'Mechanisms for ATP-dependent chromatin remodelling: farewell to the tuna-can octamer?'. Curr Opin Genet Dev, 14 (2):165-173 [Details]
	(2004)	'Sin mutations alter inherent nucleosome mobility' Flaus A, Rencurel C, Ferreira H, Wiechens N, Owen-Hughes T. ( (2004) 'Sin mutations alter inherent nucleosome mobility'. EMBO J, 23 (2):343-353 [Details]
	(2003)	'Evidence for DNA translocation by the ISWI chromatin-remodeling enzyme' Whitehouse I, Stockdale C, Flaus A, Szczelkun MD, Owen-Hughes T. (2003) 'Evidence for DNA translocation by the ISWI chromatin-remodeling enzyme'. Mol Cell Biol, 23 (6):1935-1945 [Details]
	(2003)	'Mechanisms for nucleosome mobilization' Flaus A, Owen-Hughes T. (2003) 'Mechanisms for nucleosome mobilization'. Biopolymers, 68 (4):563-578 [Details]
	(2003)	'Dynamic properties of nucleosomes during thermal and ATP-driven mobilization' Flaus A, Owen-Hughes T. (2003) 'Dynamic properties of nucleosomes during thermal and ATP-driven mobilization'. Mol Cell Biol, 23 (21):7767-7779 [Details]
	(2003)	'Histone H2A/H2B dimer exchange by ATP-dependent chromatin remodeling activities' Bruno M, Flaus A, Stockdale C, Rencurel C, Ferreira H, Owen-Hughes T. (2003) 'Histone H2A/H2B dimer exchange by ATP-dependent chromatin remodeling activities'. Mol Cell, 12 (6):1599-1606 [Details]

Conference Contributions

	Year	Publication
	(2014)	British Society for Cell Biology Spring meeting 2014, Dodson H, Eykelenboom J, Pinto D, Doran J and Flaus A (2014) BSCB Spring 2014. [Conference Paper], British Society for Cell Biology Spring meeting 2014, Warwick , 16-MAR-14 - 19-MAR-14. [Details]

Editorial

	Year	Publication
	(2019)	The Face of Chromatin Variants. Flaus, A,Owen-Hughes, T (2019) The Face of Chromatin Variants. Editorial [DOI] [Details]
	(2017)	Unlocking the nucleosome. Flaus, A;Owen-Hughes, T (2017) Unlocking the nucleosome. WASHINGTON: Editorial [DOI] [Details]

Reviews

	Year	Publication
	(2011)	Mechanisms for ATP-dependent chromatin remodelling: the means to the end. Flaus, A,Owen-Hughes, T (2011) Mechanisms for ATP-dependent chromatin remodelling: the means to the end. Reviews [DOI] [Details]
	(2006)	Chromatin modulation and the DNA damage response. Costelloe, T,FitzGerald, J,Murphy, NJ,Flaus, A,Lowndes, NF (2006) Chromatin modulation and the DNA damage response. Reviews [DOI] [Details]